Cl Anion-Dependent Mg-ATPase
نویسندگان
چکیده
منابع مشابه
Anion Permeation in Ca2+-Activated Cl− Channels
Ca(2+)-activated Cl channels (Cl(Ca)Cs) are an important class of anion channels that are opened by increases in cytosolic [Ca(2+)]. Here, we examine the mechanisms of anion permeation through Cl(Ca)Cs from Xenopus oocytes in excised inside-out and outside-out patches. Cl(Ca)Cs exhibited moderate selectivity for Cl over Na: P(Na)/P(Cl) = 0.1. The apparent affinity of Cl(Ca)Cs for Cl was low: K(...
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It has been determined that Mg2+ stimulates phosphorylation while Cl- stimulates dephosphorylation of the chloride pump during its reaction sequence. The stoichiometry of ATP hydrolyzed to Cl- transported during a single cycle of the reaction sequence was ascertained. Intracellular concentrations of ATP, ADP and inorganic phosphate were determined and, coupled with an estimate of the standard f...
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An anion-translocating ATPase has been identified as the product of the arsenical resistance operon of resistance plasmid R773. When expressed in Escherichia coli this ATP-driven oxyanion pump catalyzes extrusion of the oxyanions arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces resistance to the toxic agents. The pump is composed of two po...
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A light microsomal membrane fraction (collected on 28% weight/volume sucrose) from young mycelia of Phycomyces blakesleeanus Bgff. stationary cultures exhibited Mg(2+) ATP-dependent proton pumping as monitored with the dye neutral red or by accumulation of [(14)C]methylamine. The substrate kinetics for ATP (K(m(ATP)) 1.1 millimolar in the presence of 2 millimolar Mg(2+)), the competitive inhibi...
متن کاملHigh Affinity Ca 2 + - stimulated Mg 2 + - dependent ATPase in Rat Brain Synaptosomes , Synaptic Membranes
High affinity Ca2+-stimulated Mga+-dependent ATPase activity of nerve ending particles (!vnaphmm) from rat brain tissue appears to be associated primarily with isolated synaptic plasma membranes. The synaptic membrane (Ca2+ + Mga+)-ATPase activity was found to exhibit strict dependence on Mg+ for the presence of the activity, a high affinity for Ca2+ (&.a = 0.23 NM), and relatively high affini...
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ژورنال
عنوان ژورنال: The Journal of Membrane Biology
سال: 2012
ISSN: 0022-2631,1432-1424
DOI: 10.1007/s00232-012-9423-9